［1］ISHINO Y，SHINAGAWA H，MAKINO K，et al.Nucleotide sequence of the iap gene，responsible for alkaline phosphatase isozyme conversion in Escherichia coli，and identification of the gene product［J］.J Bacteriol，1987，169(12)：5429-5433.
［2］JANSEN R，EMBDEN J D，GAASTRA W，et al.Identification of genes that are associated with DNA repeats in prokaryotes［J］.Mol Microbiol，2002，43(6)：1565-1575.
［3］DELTCHEVA E，CHYLINSKI K，SHARMA C M，et al.CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III［J］.Nature，2011，471(7340)：602-607.
［4］JINEK M，CHYLINSKI K，FONFARA I，et al.A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity［J］. Science，2012，337(6096)：816-821.
［5］JIANG W，BIKARD D，COX D，et al.RNA-guided editing of bacterial genomes using CRISPR-Cas systems［J］.Nat Biotechnol，2013，31(3)：233-239.
［6］HWANG W Y，FU Y，REYON D，et al.Efficient genome editing in zebrafish using a CRISPR-Cas system［J］.Nat Biotechnol，2013，31(3)：227-229.
［7］CONG L，RAN F A，COX D，et al.Multiplex genome engineering using CRISPR/Cas systems［J］.Science，2013，339(6121)：819-823.
［8］WANG H，YANG H，SHIVALILA C S，et al.One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering［J］.Cell，2013，153(4)：910-918.
［9］刘志国.CRISPR/Cas9系统介导基因组编辑的研究进展［J］.畜牧兽医学报，2014，45（10）：1567-1583.
LIU Z G.Research progress on CRISPR/Cas9 mediated genome editing［J］.Acta Veterinaria et Zootechnica Sinica，2014，45(10)：1567-1583.(in Chinese)
［10］KUSCU C，ARSLAN S，SINGH R，et al.Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease［J］.Nat Biotechnol，2014，37(7)：677-683.
［11］MALI P，ESVELT K M，CHURCH G M.Cas9 as a versatile tool for engineering biology［J］. Nat Methods，2013，10(10)：957-963.
［12］REN X，YANG Z，XU J，et al.Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila［J］.Cell Rep，2014，9(3)：1151-1162.
［13］HAN H B，MA Y H，WANG T，et al.One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system［J］.Front Agr Sci Eng，2014，1(1)：2-5.
［14］LI W，TENG F，LI T，et al.Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems［J］.Nat Biotechnol，2013，31(8)：684-686.
［15］MALI P，YANG L，ESVELT K M，et al.RNA-guided human genome engineering via Cas9［J］.Science，2013，339(6121)：823-826.
［16］BASSETT A R，TIBBIT C，PONTING C P，et al.Highly efficient targeted mutagenesis of drosophila with the CRISPR/Cas 9 system［J］.Cell Rep，2013，4(1)：220-228.